Gene Expression and Drug Sensitivity Analysis of Mitochondrial Chaperones Reveals That HSPD1 and TRAP1 Expression Correlates with Sensitivity to Inhibitors of DNA Replication and Mitosis

Mitochondria—critical metabolic hubs in eukaryotic cells—are involved a wide range of cellular functions, including differentiation, proliferation, and death. Mitochondria import most their proteins from the cytosol linear form, after which they are folded by mitochondrial chaperones. However, despite extensive research, extent to function particular chaperones is essential for maintaining specific functions remains unknown. In particular, it not known whether influence sensitivity drugs used treatment cancers. By mining gene expression drug data cancer cell lines publicly available databases, we identified whose associated with oncology targeting pathways cancer-type-dependent manner. Importantly, found TRAP1 HSPD1 be inhibitors DNA replication mitosis. We confirmed experimentally that an increased ovarian cells mitosis reduced promoting apoptosis. Taken together, our results support model hinge upon provide basis understanding selectivity chaperone-substrate specificity.